In vivo estimation of pigment composition and optical absorption cross-section by spectroradiometry in four aquatic photosynthetic micro-organisms
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Non |
| Statut de publication | : | Publié |
| Année de publication | : | 2013 |
| Auteurs (8) | : | MELEDER Vona LAVIALE Martin JESUS Bruno MOUGET J,l LAVAUD Johann KAZEMIPOUR Farzaneh LAUNEAU Patrick BARILLÉ Laurent |
| Revue scientifique | : | Journal of Photochemistry and Photobiology B: Biology |
| Volume | : | 129 |
| Fascicule | : | |
| Pages | : | 115-124 |
| DOI | : | 10.1016/j.jphotobiol.2013.10.005 |
| URL | : | http://dx.doi.org/10.1016/j.jphotobiol.2013.10.005 |
| Abstract | : | The objective of the present study was to estimate in vivo pigment composition and to retrieve absorption cross-section values, a*, of photosynthetic micro-organisms using a non-invasive technique of reflectance spectrometry. To test the methodology, organisms from different taxonomical groups and different pigment composition were used (Spirulina platensis a Cyanophyta, Porphyridium cruentum a Rhodophyta, Dunaliella tertiolecta a Chlorophyta and Entomoneis paludosa a Bacillariophyta) and photoacclimated to two different irradiance levels: 25 mu mol photon m(-2) s(-1) (Low Light, LL) and 500 mu mol photon m(-2) s(-1) (High Light, HL). Second derivative spectra from reflectance were used to identify pigment in vivo absorption bands that were linked to specific pigments detected by high performance liquid chromatography. Whereas some absorption bands such as those induced by Chlorophyll (Chl) a (416, 440, 625 and around 675 nm) were ubiquous, others were taxonomically specific (e.g. 636 nm for Chl c in E. paludosa) and/or photo-physiological dependent (e.g. 489 nm for zeaxanthin in the HL-acclimated S. platensis). The optical absorption cross-section, a*, was retrieved from reflectance data using a radiative transfer model previously developed for microphytobenthos. Despite the cellular Chl a decrease observed from LL to HL (up to 88\% for S. platensis), the a* |
| Mots-clés | : | - |
| Commentaire | : | - |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Meleder V, Laviale M, Jesus B, Mouget JL, Lavaud J, Kazemipour F, Launeau P, Barillé L (2013) In vivo estimation of pigment composition and optical absorption cross-section by spectroradiometry in four aquatic photosynthetic micro-organisms. J Photochem Photobiol B, Biol 129: 115-124 | doi: 10.1016/j.jphotobiol.2013.10.005
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