Effects of Sodium Azide on the Abundance of Prokaryotes and Viruses in Marine Samples

Type : ACL
Nature : Production scientifique
Au bénéfice du Laboratoire : Oui
Statut de publication : Publié
Année de publication : 2012
Auteurs (3) : WINTER Christian KERROS Marie-emmanuelle WEINBAUER Markus,g
Revue scientifique : Plos One
Volume : 7
Fascicule : 5
Pages :
DOI : 10.1371/journal.pone.0037597
URL : <go to isi>://wos:000305343500141
Abstract : Flow cytometry is set to become the standard method for enumerating prokaryotes and viruses in marine samples. However, the samples need to be flash-frozen in liquid nitrogen directly after aldehyde fixation. Because liquid nitrogen may not always be available, we tested the potential of sodium azide as a preservative for prokaryotes and viruses in marine samples as a possible alternative. For that we conducted incubation experiments with untreated and sodium azide treated marine water samples at 4 degrees C and room temperature. The data indicate that sodium azide cannot be used to maintain marine samples used for the enumeration of prokaryotes and viruses.
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Citation :
Winter C, Kerros M-E, Weinbauer MG (2012) Effects of Sodium Azide on the Abundance of Prokaryotes and Viruses in Marine Samples. Plos One 7 | doi: 10.1371/journal.pone.0037597