Microstructural evolution and nanoscale crystallography in scleractinian coral spherulites
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Oui |
| Statut de publication | : | Publié |
| Année de publication | : | 2013 |
| Auteurs (7) | : | VAN DE LOCHT R VERCH A SAUNDERS Martin DISSARD Delphine RIXEN Tim MOYA Aurélie KRÖGER Roland |
| Revue scientifique | : | Journal of Structural Biology |
| Volume | : | 183 |
| Fascicule | : | 1 |
| Pages | : | 57-65 |
| DOI | : | 10.1016/j.jsb.2013.05.005 |
| URL | : | <go to isi>://wos:000321993700007 |
| Abstract | : | One of the most important aspects in the research on reef-building corals is the process by which corals accrete biogenic calcium carbonate. This process leads to the formation of a mineral/organic composite and it is believed that the development of the nano- and microstructure of the mineral phase is highly sensitive to the growth conditions. Transmission electron microscopy (TEM) analysis of large-scale (10 x 30 mu m) focused ion beam (FIB) prepared lamellae was performed on adult and juvenile scleractinian coral skeleton specimens. This allowed for the investigation of the nano and microstructure and the crystallographic orientation of the aragonite mineral. We found the following microstructural evolution in the adult Porites lobate specimens: randomly oriented nanocrystals with high porosity, partly aligned nanocrystals with high porosity and areas of dense acicular crystals of several micrometers extension, the latter two areas are aligned close to the [001] direction (Pmcn space group). To the best of our knowledge, for the first time the observed microstructure could be directly correlated with the dark/bright bands characteristic of the diurnal growth cycle. We hypothesize that this mineral structure sequence and alignment in the adult specimen is linked to the photosynthetic diurnal cycle of the zooxanthellea regulating the oxygen levels and organic molecule transport to the calcifying medium. These observations reveal a strong control of crystal morphology by the organism and the correlation of the accretion process. No indication for a self-assembly of nanocrystalline units, i.e., a mesocrystal structure, on the micrometer scale could be found. (C) 2013 Elsevier Inc. All rights reserved. |
| Mots-clés | : | Biomineralization Transmission electron microscopy Aragonite Crystallography growth biomineralization calcification skeletons calcite acidification organization crystals impact sea Biochemistry & Molecular Biology Biophysics Cell Biology |
| Commentaire | : | ISI Document Delivery No.: 185TR Times Cited: 3 Cited Reference Count: 42 van de Locht, Renee Verch, Andreas Saunders, Martin Dissard, Delphine Rixen, Tim Moya, Aurelie Kroeger, Roland Saunders, Martin/B-3082-2011; Kroeger, Roland/D-5321-2012 Saunders, Martin/0000-0001-6873-7816; EPSRC [EP/I001514/1]; University of York; Australian Research Council (ARC) [DP0986505]; ARC Centre of Excellence in Coral Reef Studies; Marie Curie International Outgoing Fellowship We would like to thank EPSRC (Grant no. EP/I001514/1) for funding and the York JEOL Nanocentre for the use of their facilities. The work was supported by an International Seedcorn award from the University of York. We are grateful to the Leeds EPSRC Nanoscience and Nanotechnology Research Equipment Facility for access to the FIB system. The authors acknowledge the facilities, and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy, Characterisation & Analysis. The University of Western Australia, a facility funded by the University, State and Commonwealth Governments. We would also like to thank the Australian Research Council (ARC) grant DP0986505 and the ARC Centre of Excellence in Coral Reef Studies for financial support to Delphine Dissard and we would like to acknowledge the support of the Marie Curie International Outgoing Fellowship to Aurelie Moya. 3 Academic press inc elsevier science San diego Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Van de Locht R, Verch A, Saunders M, Dissard D, Rixen T, Moya A, Kröger R (2013) Microstructural evolution and nanoscale crystallography in scleractinian coral spherulites. J Struct Biol 183: 57-65 | doi: 10.1016/j.jsb.2013.05.005
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