Development of phoH as a Novel Signature Gene for Assessing Marine Phage Diversity

Type : ACL
Nature : Production scientifique
Au bénéfice du Laboratoire : Oui
Statut de publication : Publié
Année de publication : 2011
Auteurs (9) : GOLDSMITH D,b CROSTI Giuseppe DWIVEDI B MCDANIEL L,d VARSANI A SUTTLE Curtis,a WEINBAUER Markus,g SANDAA Ruth-anne BREITBART Mya
Revue scientifique : Applied And Environmental Microbiology
Volume : 77
Fascicule : 21
Pages : 7730-7739
DOI : 10.1128/aem.05531-11
URL : <go to isi>://wos:000296568200034
Abstract : Phages play a key role in the marine environment by regulating the transfer of energy between trophic levels and influencing global carbon and nutrient cycles. The diversity of marine phage communities remains difficult to characterize because of the lack of a signature gene common to all phages. Recent studies have demonstrated the presence of host-derived auxiliary metabolic genes in phage genomes, such as those belonging to the Pho regulon, which regulates phosphate uptake and metabolism under low-phosphate conditions. Among the completely sequenced phage genomes in GenBank, this study identified Pho regulon genes in nearly 40% of the marine phage genomes, while only 4% of nonmarine phage genomes contained these genes. While several Pho regulon genes were identified, phoH was the most prevalent, appearing in 42 out of 602 completely sequenced phage genomes. Phylogenetic analysis demonstrated that phage phoH sequences formed a cluster distinct from those of their bacterial hosts. PCR primers designed to amplify a region of the phoH gene were used to determine the diversity of phage phoH sequences throughout a depth profile in the Sargasso Sea and at six locations worldwide. phoH was present at all sites examined, and a high diversity of phoH sequences was recovered. Most phoH sequences belonged to clusters without any cultured representatives. Each depth and geographic location had a distinct phoH composition, although most phoH clusters were recovered from multiple sites. Overall, phoH is an effective signature gene for examining phage diversity in the marine environment.
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Commentaire : Times Cited: 13 Breitbart, Mya/B-1366-2009; Suttle, Curtis/C-3150-2008 Suttle, Curtis/0000-0002-0372-0033 14
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Citation :
Goldsmith DB, Crosti G, Dwivedi B, McDaniel LD, Varsani A, Suttle CA, Weinbauer MG, Sandaa R-A, Breitbart M (2011) Development of phoH as a Novel Signature Gene for Assessing Marine Phage Diversity. Appl Environ Microb 77: 7730-7739 | doi: 10.1128/aem.05531-11