Lack of P-limitation of phytoplankton and heterotrophic prokaryotes in surface waters of three anticyclonic eddies in the stratified Mediterranean Sea
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Non |
| Statut de publication | : | Publié |
| Année de publication | : | 2011 |
| Auteurs (13) | : | TANAKA Tsuneo THINGSTAD T,f CHRISTAKI Urania COLOMBET J CORNET-BARTHAUX V COURTIES C GRATTEPANCHE J-d LAGARIA A NEDOMA J ORIOL L PSARRA S PUJO-PAY Mireille VAN WAMBEKE France |
| Revue scientifique | : | Biogeosciences |
| Volume | : | 8 |
| Fascicule | : | 2 |
| Pages | : | 525-538 |
| DOI | : | 10.5194/bg-8-525-2011 |
| URL | : | <go to isi>://wos:000287796800021 |
| Abstract | : | We investigated the identity of the limiting nutrient of the pelagic microbial food web in the Mediterranean Sea using nutrient manipulated microcosms during summer 2008. Experiments were carried out with surface waters at the center of anticyclonic eddies in the Western Basin, the Ionian Basin, and the Levantine Basin. In situ, the ratio of N to P was always higher in both dissolved and particulate organic fractions compared to the Redfield ratio, suggesting a relative P-starvation. In each experiment, four different treatments in triplicates (addition of ammonium, phosphate, a combination of both, and the unamended control) were employed and chemical and biological parameters monitored throughout a 3-4 day incubation. Temporal changes of turnover time of phosphate and ATP, and alkaline phosphatase activity during the incubation suggested that the phytoplankton and heterotrophic prokaryotes (Hprok) communities were not P-limited at the sites. Furthermore, statistical comparison among treatments at the end of the incubation did not support a hypothesis of P-limitation at the three study sites. In contrast, primary production was consistently limited by N, and Hprok growth was not limited by N nor P in the Western Basin, but N-limited in the Ionian Basin, and N and P co-limited in the Levantine Basin. Our results demonstrated the gap between biogeochemical features (an apparent P-starved status) and biological responses (no apparent P-limitation). We question the general notion that Mediterranean surface waters are limited by P alone during the stratified period. |
| Mots-clés | : | alkaline-phosphatase activity dissolved organic-carbon microbial food-web nutrient limitation villefranche bay flow-cytometry limited phytoplankton phosphorus limitation levantine basin nitrogen Environmental Sciences & Ecology Geology |
| Commentaire | : | ISI Document Delivery No.: 727KF Times Cited: 22 Cited Reference Count: 67 Tanaka, T. Thingstad, T. F. Christaki, U. Colombet, J. Cornet-Barthaux, V. Courties, C. Grattepanche, J. -D. Lagaria, A. Nedoma, J. Oriol, L. Psarra, S. Pujo-Pay, M. Van Wambeke, F. Pujo-Pay, Mireille/E-9547-2011; Tanaka, Tsuneo/G-3751-2011; Grattepanche, Jean-David/C-7101-2013; Nedoma, Jiri/F-9063-2014 Grattepanche, Jean-David/0000-0002-1854-3762; CNRS-INSU; French national LEFE-CYBER program; European commission SESAME IP The publication of this article is financed by CNRS-INSU.This is a contribution to the BOUM (Biogeochemistry from the Oligotrophic to the Ultraoligotrophic Mediterranean) project financed by the French national LEFE-CYBER program and the European commission SESAME IP. The BOUM project was coordinated by the Institut des Sciences de l'Univers (INSU) and managed by the Centre National de la Recherche Scientifique (CNRS). The authors thank the crew of the R/V L'Atalante for outstanding shipboard operation, chief scientists T. Moutin and L. Prieur for the cruise organization, C. Marec and L. Prieur for CTD and rosette operation, and D. Lef` evre, M. Le Moal, A. Nowaczyk, and K. Popendorf for microcosm setup and sampling. Thanks are also due to two reviewers for useful comments, and J. Dolan for the linguistic correction. 22 Copernicus gesellschaft mbh Gottingen Ecology; Geosciences, Multidisciplinary |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Tanaka T, Thingstad TF, Christaki U, Colombet J, Cornet-Barthaux V, Courties C, Grattepanche J-D, Lagaria A, Nedoma J, Oriol L, Psarra S, Pujo-Pay M, Van Wambeke F (2011) Lack of P-limitation of phytoplankton and heterotrophic prokaryotes in surface waters of three anticyclonic eddies in the stratified Mediterranean Sea. Biogeosciences 8: 525-538 | doi: 10.5194/bg-8-525-2011
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