Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Oui |
| Statut de publication | : | Publié |
| Année de publication | : | 2017 |
| Auteurs (50) | : | ALBERTI Adriana POULAIN Julie ENGELEN Stefan LABADIE Karine ROMAC Sarah FERRERA Isabel ALBINI Guillaume AURY Jean-marc BELSER Caroline BERTRAND Alexis CRUAUD Corinne DA SILVA Corinne DOSSAT Carole GAVORY Frédérick GAS Shahinaz GUY Julie HAQUELLE Maud JACOBY E'krame JAILLON Olivier LEMAINQUE Arnaud PELLETIER Eric SAMSON Gaëlle WESSNER Mark GENOSCOPE TECHNICAL Team ACINAS Silvia,g ROYO-LLONCH Marta CORNEJO-CASTILLO Francisco,m LOGARES Ramiro FERNÁNDEZ-GÓMEZ Beatriz BOWLER Chris COCHRANE Guy AMID Clara HOOPEN Petra ten DE VARGAS Colomban GRIMSLEY Nigel DESGRANGES Elodie KANDELS-LEWIS Stefanie OGATA Hiroyuki POULTON Nicole SIERACKI Michael,e STEPANAUSKAS Ramunas SULLIVAN Matthew,b BRUM Jennifer,r DUHAIME Melissa,b POULOS Bonnie,t HURWITZ Bonnie,l TARA OCEANS CONSORTIUM Coordinators PESANT Stéphane KARSENTI Eric WINCKER Patrick |
| Revue scientifique | : | Scientific Data |
| Volume | : | 4 |
| Fascicule | : | 65535 |
| Pages | : | |
| DOI | : | 10.1038/sdata.2017.93 |
| URL | : | http://dx.doi.org/10.1038/sdata.2017.93 |
| Abstract | : | A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009–2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world’s planktonic ecosystems. |
| Mots-clés | : | Metagenomics ; Molecular biology ; Water microbiology |
| Commentaire | : | - |
| Tags | : | TARA |
| Fichier attaché | : | - |
| Citation | : |
Alberti A, Poulain J, Engelen S, Labadie K, Romac S, Ferrera I, Albini G, Aury J-M, Belser C, Bertrand A, Cruaud C, Da Silva C, Dossat C, Gavory F, Gas S, Guy J, Haquelle M, Jacoby E, Jaillon O, Lemainque A, Pelletier E, Samson G, Wessner M, Genoscope Technical T, Acinas SG, Royo-Llonch M, Cornejo-Castillo FM, Logares R, Fernández-Gómez B, Bowler C, Cochrane G, Amid C, Hoopen P, De Vargas C, Grimsley N, Desgranges E, Kandels-Lewis S, Ogata H, Poulton N, Sieracki ME, Stepanauskas R, Sullivan MB, Brum JR, Duhaime MB, Poulos BT, Hurwitz BL, Tara Oceans Consortium C, Pesant S, Karsenti E, Wincker P (2017) Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition. Sci Data 4 | doi: 10.1038/sdata.2017.93
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