Interactive effects of light and temperature on pigments and n-3 LC-PUFA-enriched oil accumulation in batch-cultivated Pavlova lutheri using high-bicarbonate supply
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Non |
| Statut de publication | : | Publié |
| Année de publication | : | 2017 |
| Auteurs (2) | : | GUIHÉNEUF Freddy STENGEL Dagmar,b |
| Revue scientifique | : | Algal Research |
| Volume | : | 23 |
| Fascicule | : | |
| Pages | : | 113-125 |
| DOI | : | 10.1016/j.algal.2017.02.002 |
| URL | : | - |
| Abstract | : | We investigated the simultaneous effects of light and temperature on pigments, lipid remodeling, and omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) partitioning into lipid classes of P. lutheri. Biomasswas produced in batch cultivation using high bicarbonate supply, and hence, any potential accumulation of lipid and triacylglycerols (TAG) containing n-3 LC-PUFA was triggered by nitrogen (N) limitation. The maximum productivities of both eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were obtained after nitrate-depletionwhen P. lutheriwas cultivated at optimal growth conditions; these occurred at an intermediate temperature of 18 degrees C and lowlight intensity (40 mu mol photons . m(-2).s(-1)) for EPA, but therewas no impact of light (40 or 200 mu mol photons . m(-2).s(-1)) for DHA production. Under these respective optimum conditions, TAG accounted for 82-84\% of TFA on day 15 of cultivation, which contained up to 76-84\% of the total cellular EPA and 67-81\% of total cellular DHA. Our results clearly demonstrate the process of lipid remodeling in P. lutheri and, for the first time, a potential membrane lipid turnover with transfer of n-3 LC-PUFA (EPA and DHA) from membranes (polar lipids, PL) to storage lipids (TAG), highlighting the accumulation of n-3 LC-PUFA-rich oil during N-starvation (i.e., TAG containing: EPA similar to 12-13\% and DHA similar to 5-6\% of TFA). When cultivated under low light conditions, P. lutheri additionally accumulated substantial quantities of antioxidant pigments (i.e., fucoxanthin, diadinoxanthin and beta-carotene), which add value to extracted bioactive oil for functional food applications. Omega-3 rich oil accumulation and pigment levels in P. lutheri appear to be simultaneously regulated by both light and temperature, in addition to N-limitation during batch-cultivation. (C) 2017 Elsevier B.V. All rights reserved. |
| Mots-clés | : | Pavlova lutheri; Light, temperature; N-3 LC-PUFA; Lipid remodeling and turnover; Antioxidant pigments |
| Commentaire | : | - |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Guihéneuf F, Stengel DB (2017) Interactive effects of light and temperature on pigments and n-3 LC-PUFA-enriched oil accumulation in batch-cultivated Pavlova lutheri using high-bicarbonate supply. Algal Res 23: 113-125 | doi: 10.1016/j.algal.2017.02.002
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