The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Non |
| Statut de publication | : | Publié |
| Année de publication | : | 2015 |
| Auteurs (8) | : | ROBERTSON Ruairi,c GUIHÉNEUF Freddy BAHAR Bojlul SCHMID Matthias STENGEL Dagmar,b FITZGERALD Gerald,f ROSS R,paul STANTON Catherine |
| Revue scientifique | : | Marine Drugs |
| Volume | : | 13 |
| Fascicule | : | 8 |
| Pages | : | 5402-5424 |
| DOI | : | 10.3390/md13085402 |
| URL | : | - |
| Abstract | : | Algae contain a number of anti-inflammatory bioactive compounds such as omega-3 polyunsaturated fatty acids (n-3 PUFA) and chlorophyll a, hence as dietary ingredients, their extracts may be effective in chronic inflammation-linked metabolic diseases such as cardiovascular disease. In this study, anti-inflammatory potential of lipid extracts from three red seaweeds (Porphyra dioica, Palmaria palmata and Chondrus crispus) and one microalga (Pavlova lutheri) were assessed in lipopolysaccharide (LPS)-stimulated human THP-1 macrophages. Extracts contained 34\%-42\% total fatty acids as n-3 PUFA and 5\%-7\% crude extract as pigments, including chlorophyll a, -carotene and fucoxanthin. Pretreatment of the THP-1 cells with lipid extract from P. palmata inhibited production of the pro-inflammatory cytokines interleukin (IL)-6 (p < 0.05) and IL-8 (p < 0.05) while that of P. lutheri inhibited IL-6 (p < 0.01) production. Quantitative gene expression analysis of a panel of 92 genes linked to inflammatory signaling pathway revealed down-regulation of the expression of 14 pro-inflammatory genes (TLR1, TLR2, TLR4, TLR8, TRAF5, TRAF6, TNFSF18, IL6R, IL23, CCR1, CCR4, CCL17, STAT3, MAP3K1) by the lipid extracts. The lipid extracts effectively inhibited the LPS-induced pro-inflammatory signaling pathways mediated via toll-like receptors, chemokines and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) signaling molecules. These results suggest that lipid extracts from P. lutheri, P. palmata, P. dioica and C. crispus can inhibit LPS-induced inflammatory pathways in human macrophages. Therefore, algal lipid extracts should be further explored as anti-inflammatory ingredients for chronic inflammation-linked metabolic diseases. |
| Mots-clés | : | microalgae; macroalgae; THP-1; inflammation; lipids; n-3 PUFA; polyunsaturated fatty acids; macrophages; chlorophyll a; bioactive pigments |
| Commentaire | : | - |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Robertson RC, Guihéneuf F, Bahar B, Schmid M, Stengel DB, Fitzgerald GF, Ross RP, Stanton C (2015) The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages. Mar Drugs 13: 5402-5424 | doi: 10.3390/md13085402
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