Light Intensity Regulates LC-PUFA Incorporation into Lipids of Pavlova lutheri and the Final Desaturase and Elongase Activities Involved in Their Biosynthesis
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Non |
| Statut de publication | : | Publié |
| Année de publication | : | 2015 |
| Auteurs (4) | : | GUIHÉNEUF Freddy MIMOUNI Virginie TREMBLIN Gerard ULMANN Lionel |
| Revue scientifique | : | Journal of agricultural and food chemistry |
| Volume | : | 63 |
| Fascicule | : | 4 |
| Pages | : | 1261-1267 |
| DOI | : | 10.1021/jf504863u |
| URL | : | - |
| Abstract | : | The microalga Pavlova lutheri is a candidate for the production of omega-3 long-chain polyunsaturated fatty acid (LC-PUFA), due to its ability to accumulate both eicosapentaenoic (EPA) and docosahexaenoic acids. Outstanding questions need to be solved to understand the complexity of n-3 LC-PUFA synthesis and partitioning into lipids, especially its metabolic regulation, and which enzymes and/or abiotic factors control their biosynthesis. In this study, the radioactivity of 14C-labeled arachidonic acid incorporated into the total lipids of P. lutheri grown under different light intensities and its conversion into labeled LC-PUFA were monitored. The results highlighted for the first time the light-dependent incorporation of LC-PUFA into lipids and the light-dependent activity of the final desaturation and elongation steps required to synthesize and accumulate n-3 C20/C22 LC-PUFA. The incorporation of arachidonic acid into lipids under low light and the related Delta 17-desaturation activity measured explain the variations in fatty acid profile of P. lutheri, especially the accumulation of n-3 LC-PUFA such as EPA under low light conditions. |
| Mots-clés | : | Pavlova lutheri; marine microalga; in vivo C-14-labeling; n-3 LC-PUFA biosynthesis; LC-PUFA incorporation; Delta 17-desaturation; light intensity |
| Commentaire | : | - |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Guihéneuf F, Mimouni V, Tremblin G, Ulmann L (2015) Light Intensity Regulates LC-PUFA Incorporation into Lipids of Pavlova lutheri and the Final Desaturase and Elongase Activities Involved in Their Biosynthesis. J Agric Food Chem 63: 1261-1267 | doi: 10.1021/jf504863u
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