Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Oui |
| Statut de publication | : | Publié |
| Année de publication | : | 2021 |
| Auteurs (21) | : | POLERECKY Lubos MASUDA T EICHNER Meri RABOUILLE Sophie VANCOVÁ Marie KIENHUIS Michiel,v,m BERNÁT G BONOMI-BARUFI Jose CAMPBELL Douglas,andrew CLAQUIN Pascal ČERVENÝ Jan GIORDANO Mario KOTABOVÁ E KROMKAMP Jacco LOMBARDI Ana-teresa LUKEŠ Martin PRASIL O STEPHAN Susanne SUGGETT D ZAVŘEL Tomas HALSEY L,g |
| Revue scientifique | : | Frontiers in Microbiology |
| Volume | : | 12 |
| Fascicule | : | |
| Pages | : | |
| DOI | : | 10.3389/fmicb.2021.620915 |
| URL | : | https://www.frontiersin.org/article/10.3389/fmicb.2021.620915 |
| Abstract | : | Unicellular nitrogen fixing cyanobacteria (UCYN) are abundant members of phytoplankton communities in a wide range of marine environments, including those with rapidly changing nitrogen (N) concentrations. We hypothesized that differences in N availability (N2 vs. combined N) would cause UCYN to shift strategies of intracellular N and C allocation. We used transmission electron microscopy and nanoscale secondary ion mass spectrometry imaging to track assimilation and intracellular allocation of 13C-labeled CO2 and 15N-labeled N2 or NO3 at different periods across a diel cycle in Cyanothece sp. ATCC 51142. We present new ideas on interpreting these imaging data, including the influences of pre-incubation cellular C and N contents and turnover rates of inclusion bodies. Within cultures growing diazotrophically, distinct subpopulations were detected that fixed N2 at night or in the morning. Additional significant within-population heterogeneity was likely caused by differences in the relative amounts of N assimilated into cyanophycin from sources external and internal to the cells. Whether growing on N2 or NO3, cells prioritized cyanophycin synthesis when N assimilation rates were highest. N assimilation in cells growing on NO3 switched from cyanophycin synthesis to protein synthesis, suggesting that once a cyanophycin quota is met, it is bypassed in favor of protein synthesis. Growth on NO3 also revealed that at night, there is a very low level of CO2 assimilation into polysaccharides simultaneous with their catabolism for protein synthesis. This study revealed multiple, detailed mechanisms underlying C and N management in Cyanothece that facilitate its success in dynamic aquatic environments. |
| Mots-clés | : | - |
| Commentaire | : | - |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Polerecky L, Masuda T, Eichner M, Rabouille S, Vancová M, Kienhuis MVM, Bernát G, Bonomi-Barufi J, Campbell DA, Claquin P, Červený J, Giordano M, Kotabová E, Kromkamp J, Lombardi A-T, Lukeš M, Prasil O, Stephan S, Suggett D, Zavřel T, Halsey LG (2021) Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142. Front Microbiol 12 | doi: 10.3389/fmicb.2021.620915
|