Toxicity of palytoxin, purified ovatoxin-a, ovatoxin-d and extracts of Ostreopsis cf. ovata on the Caco-2 intestinal barrier model
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Oui |
| Statut de publication | : | Publié |
| Année de publication | : | 2022 |
| Auteurs (9) | : | GÉMIN Marin-pierre LANCELEUR Rachelle MESLIER Lisa HERVÉ Fabienne RÉVEILLON Damien AMZIL Zouher TERNON Eva THOMAS Olivier,p FESSARD V |
| Revue scientifique | : | Environmental Toxicology and Pharmacology |
| Volume | : | |
| Fascicule | : | |
| Pages | : | |
| DOI | : | 10.1016/j.etap.2022.103909 |
| URL | : | https://www.sciencedirect.com/science/article/pii/S1382668922001028 |
| Abstract | : | Human intoxications in the Mediterranean Sea have been linked to blooms of the dinoflagellate Ostreopsis cf. ovata, producer of palytoxin (PlTX)-like toxins called ovatoxins (OVTXs). Exposure routes include only inhalation and contact, although PlTX-poisoning by seafood has been described in tropical regions. To address the impact of OVTXs on the intestinal barrier, dinoflagellate extracts, purified OVTX-a and -d and PlTX were tested on differentiated Caco-2 cells. Viability, inflammatory response and barrier integrity were recorded after 24h treatment. OVTX-a and -d were not cytotoxic up to 20ng/mL but increased IL-8 release, although to a lesser extent compared to PlTX. While PlTX and OVTX-a (at 0.5 and 5ng/mL respectively) affected intestinal barrier integrity OVTX-d up to 5ng/mL did not. Overall, OVTX-d was shown to be less toxic than OVTX-a and PlTX. Therefore, oral exposure to OVTX-a and -d could provoked lower acute toxicity than PlTX. |
| Mots-clés | : | inflammation; ovatoxins; palytoxin; permeability; purification; toxicity |
| Commentaire | : | - |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Gémin M-P, Lanceleur R, Meslier L, Hervé F, Réveillon D, Amzil Z, Ternon E, Thomas OP, Fessard V (2022) Toxicity of palytoxin, purified ovatoxin-a, ovatoxin-d and extracts of Ostreopsis cf. ovata on the Caco-2 intestinal barrier model. Environ Toxicol Pharmacol | doi: 10.1016/j.etap.2022.103909
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