Impact of Carbon Source Supplementations on Pseudomonas aeruginosa Physiology
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Non |
| Statut de publication | : | Publié |
| Année de publication | : | 2022 |
| Auteurs (12) | : | SAUVAGE S GAVIARD Charlotte TAHRIOUI Ali COQUET Laurent LE Hung ALEXANDRE Stéphane BEN ABDELKRIM Ahmed BOUFFARTIGUES Emeline LESOUHAITIER Olivier CHEVALIER Sylvie JOUENNE Thierry HARDOUIN Julie |
| Revue scientifique | : | Journal of Proteome Research |
| Volume | : | 21 |
| Fascicule | : | 6 |
| Pages | : | 1392-1407 |
| DOI | : | 10.1021/acs.jproteome.1c00936 |
| URL | : | https://doi.org/10.1021/acs.jproteome.1c00936 |
| Abstract | : | Pseudomonas aeruginosa is an opportunistic pathogen highly resistant to a wide range of antimicrobial agents, making its infections very difficult to treat. Since microorganisms need to perpetually adapt to their surrounding environment, understanding the effect of carbon sources on P. aeruginosa physiology is therefore essential to avoid increasing drug-resistance and better fight this pathogen. By a global proteomic approach and phenotypic assays, we investigated the impact of various carbon source supplementations (glucose, glutamate, succinate, and citrate) on the physiology of the P. aeruginosa PA14 strain. A total of 581 proteins were identified as differentially expressed in the 4 conditions. Most of them were more abundant in citrate supplementation and were involved in virulence, motility, biofilm development, and antibiotic resistance. Phenotypic assays were performed to check these hypotheses. By coupling all this data, we highlight the importance of the environment in which the bacterium evolves on its metabolism, and thus the necessity to better understand the metabolic pathways implied in its adaptative response according to the nutrient availability. |
| Mots-clés | : | - |
| Commentaire | : | - |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Sauvage S, Gaviard C, Tahrioui A, Coquet L, Le H, Alexandre S, Ben Abdelkrim A, Bouffartigues E, Lesouhaitier O, Chevalier S, Jouenne T, Hardouin J (2022) Impact of Carbon Source Supplementations on Pseudomonas aeruginosa Physiology. J Proteome Res 21: 1392-1407 | doi: 10.1021/acs.jproteome.1c00936
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