Global distribution patterns of marine nitrogen-fixers by imaging and molecular methods
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Oui |
| Statut de publication | : | Publié |
| Année de publication | : | 2021 |
| Auteurs (15) | : | PIERELLA KARLUSICH Juan,j PELLETIER Eric LOMBARD Fabien CARSIQUE Madeline DVORAK Etienne COLIN Sébastien PICHERAL Marc CORNEJO-CASTILLO Francisco,m ACINAS Silvia,g PEPPERKOK Rainer KARSENTI Eric DE VARGAS Colomban WINCKER Patrick BOWLER Chris FOSTER R,a |
| Revue scientifique | : | Nature Communications |
| Volume | : | 12 |
| Fascicule | : | 1 |
| Pages | : | |
| DOI | : | 10.1038/s41467-021-24299-y |
| URL | : | https://doi.org/10.1038/s41467-021-24299-y |
| Abstract | : | Nitrogen fixation has a critical role in marine primary production, yet our understanding of marine nitrogen-fixers (diazotrophs) is hindered by limited observations. Here, we report a quantitative image analysis pipeline combined with mapping of molecular markers for mining {\textgreater}2,000,000 images and {\textgreater}1300 metagenomes from surface, deep chlorophyll maximum and mesopelagic seawater samples across 6 size fractions ({\textless}0.2–2000 μm). We use this approach to characterise the diversity, abundance, biovolume and distribution of symbiotic, colony-forming and particle-associated diazotrophs at a global scale. We show that imaging and PCR-free molecular data are congruent. Sequence reads indicate diazotrophs are detected from the ultrasmall bacterioplankton ({\textless}0.2 μm) to mesoplankton (180–2000 μm) communities, while images predict numerous symbiotic and colony-forming diazotrophs ({\textgreater}20 µm). Using imaging and molecular data, we estimate that polyploidy can substantially affect gene abundances of symbiotic versus colony-forming diazotrophs. Our results support the canonical view that larger diazotrophs ({\textgreater}10 μm) dominate the tropical belts, while unicellular cyanobacterial and non-cyanobacterial diazotrophs are globally distributed in surface and mesopelagic layers. We describe co-occurring diazotrophic lineages of different lifestyles and identify high-density regions of diazotrophs in the global ocean. Overall, we provide an update of marine diazotroph biogeographical diversity and present a new bioimaging-bioinformatic workflow. |
| Mots-clés | : | - |
| Commentaire | : | - |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Pierella Karlusich JJ, Pelletier E, Lombard F, Carsique M, Dvorak E, Colin S, Picheral M, Cornejo-Castillo FM, Acinas SG, Pepperkok R, Karsenti E, De Vargas C, Wincker P, Bowler C, Foster RA (2021) Global distribution patterns of marine nitrogen-fixers by imaging and molecular methods. Nat Commun 12 | doi: 10.1038/s41467-021-24299-y
|