Quantifying Per-Cell Chlorophyll a in Natural Picophytoplankton Populations Using Fluorescence-Activated Cell Sorting
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Non |
| Statut de publication | : | Publié |
| Année de publication | : | 2022 |
| Auteurs (5) | : | BOCK Nicholas SUBRAMANIAM Ajit JUHL A,r MONTOYA J DUHAMEL S |
| Revue scientifique | : | Frontiers in Marine Science |
| Volume | : | 9 |
| Fascicule | : | |
| Pages | : | |
| DOI | : | 10.3389/fmars.2022.850646 |
| URL | : | - |
| Abstract | : | Marine phytoplankton play a central role in global biogeochemical cycling, carbon export, and the overall functioning of marine ecosystems. While chlorophyll a (Chl a) is widely used as a proxy for phytoplankton biomass, identifying the proportion of Chl a attributable to diferent phytoplankton groups remains a major challenge in oceanography, especially for the picophytoplankton groups that often represent the majority of phytoplankton biomass in the open ocean. We describe a method for measuring picophytoplankton per-cell Chl a in field samples using fluorescence-activated cell sorting followed by solvent-based Chl a extraction and fluorescence quantification. Applying this method to surface samples from the Gulf of Mexico, we determined per-cell Chl a to be 0.24 +/- 0.07, 0.6 +/- 0.33, and 26.36 +/- 20.9 fg Chl a cell(-1) for Prochlorococcus, Synechococcus, and PPE, respectively (mean +/- SD). Measurements of per-cell Chl a using this method are precise to within 1.7, 2.1, and 3.1% for Prochlorococcus, Synechococcus, and PPE, respectively. We demonstrate that this approach can be used to obtain estimates of group-specific Chl a for Prochlorococcus, Synechococcus, and picophytoeukaryotes, the latter two of which cannot be captured by existing methods. We also demonstrate that measurements of per-cell Chl a made using this method in field samples are sufficiently precise to capture relationships between per-cell Chl a and cytometer red fluorescence, providing a bridge between biomass estimates from cell counts and bulk measurements of total Chl a. |
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| Commentaire | : | - |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Bock N, Subramaniam A, Juhl AR, Montoya J, Duhamel S (2022) Quantifying Per-Cell Chlorophyll a in Natural Picophytoplankton Populations Using Fluorescence-Activated Cell Sorting. Front Mar Sci 9 | doi: 10.3389/fmars.2022.850646
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