Integrating wet stirred-bead milling for Tetraselmis suecica biorefinery: Operating parameters influence and specific energy efficiency

Type : ACL
Nature : Production scientifique
Au bénéfice du Laboratoire : Non
Statut de publication : Publié
Année de publication : 2024
Auteurs (6) : DELRAN P BARTHE L PEYDECASTAING J PONTALIER P,y GUIHÉNEUF Freddy FRANCES C
Revue scientifique : Bioresource Technology
Volume : 394
Fascicule :
Pages :
DOI : 10.1016/j.biortech.2023.130181
URL : https://www-sciencedirect-com.insu.bib.cnrs.fr/science/article/pii/S0960852423016097?via%3Dihub
Abstract : Stirred bead milling proved to be an efficient cell destruction technique in a biorefinery unit for the extraction of over 95 \% of proteins and 60 \% of carbohydrates from the green marine microalga Tetraselmis suecica. Optimum conditions, expressed in terms of metabolite yield and energy consumption, were found for average values of bead size and agitator rotation speed. The higher the microalgae concentration, up to 100 g.L-1, which is adequate for biofilm algae growth in an industrial unit, the more efficient the cell destruction process. Cell destruction rates and metabolite extraction yields are similar in pendular and recycling modes, but the pendular configuration reduces the residence time of the suspension in the grinding chamber, which is less costly. With regard to the cell destruction mechanism, it was concluded that bead shocks first damage cells by permeabilizing them, and that after a longer period, all cells are shredded and destroyed, forming elongated debris.
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Delran P, Barthe L, Peydecastaing J, Pontalier PY, Guihéneuf F, Frances C (2024) Integrating wet stirred-bead milling for Tetraselmis suecica biorefinery: Operating parameters influence and specific energy efficiency. Bioresource Technol 394 | doi: 10.1016/j.biortech.2023.130181