Fatuamide A, a Hybrid PKS/NRPS Metallophore from a Leptolyngbya sp. Marine Cyanobacterium Collected in American Samoa
| Type | : | ACL |
|---|---|---|
| Nature | : | Production scientifique |
| Au bénéfice du Laboratoire | : | Oui |
| Statut de publication | : | Publié |
| Année de publication | : | 2025 |
| Auteurs (16) | : | ALEXANDER Kelsey,l NAMAN C,benjamin IWASAKI Arihiro MANGONI Alfonso LEAO Tiago REHER Raphael PETRAS Daniel KIM Hyunwoo TERNON Eva CARO-DIAZ Eduardo,j,e GLUKHOV Evgenia MITREVSKA Jana,a AVALON Nicole,e DUGGAN Brendan,m GERWICK Lena GERWICK William,h |
| Revue scientifique | : | Journal of Natural Products |
| Volume | : | |
| Fascicule | : | |
| Pages | : | |
| DOI | : | 10.1021/acs.jnatprod.4c01051 |
| URL | : | https://doi.org/10.1021/acs.jnatprod.4c01051 |
| Abstract | : | A structurally novel metabolite, fatuamide A (1), was discovered from a laboratory cultured strain of the marine cyanobacterium Leptolyngbya sp., collected from Faga’itua Bay, American Samoa. A bioassay-guided approach using NCI-H460 human lung cancer cells directed the isolation of fatuamide A, which was obtained from the most cytotoxic fraction. The planar structure of fatuamide A was elucidated by integrated NMR and MS/MS analysis, and a combination of bioinformatic and computational approaches was used to deduce the absolute configuration at its eight stereocenters. A putative hybrid PKS/NRPS biosynthetic gene cluster responsible for fatuamide A production was identified from the sequenced genomic DNA of the cultured cyanobacterium. The biosynthetic gene cluster possessed elements that suggested fatuamide A binds metals, and this metallophore property was demonstrated by native metabolomics and indicated a preference for binding copper. The producing strain was found to be highly resistant to toxicity from elevated copper concentrations in culture media. |
| Mots-clés | : | - |
| Commentaire | : | doi: 10.1021/acs.jnatprod.4c01051 |
| Tags | : | - |
| Fichier attaché | : | - |
| Citation | : |
Alexander KL, Naman CB, Iwasaki A, Mangoni A, Leao T, Reher R, Petras D, Kim H, Ternon E, Caro-Diaz EJE, Glukhov E, Mitrevska JA, Avalon NE, Duggan BM, Gerwick L, Gerwick WH (2025) Fatuamide A, a Hybrid PKS/NRPS Metallophore from a Leptolyngbya sp. Marine Cyanobacterium Collected in American Samoa. J Nat Prod | doi: 10.1021/acs.jnatprod.4c01051
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